Packaging Academy, Pet Food
Freeze-Dried vs Jerky Pet Treats: What Really Controls Shelf Life—Water Activity or Oxygen?
Pet treats can look “dry” and still fail fast on shelf. Brands often guess wrong about what is actually limiting: microbes, rancidity, or texture drift.
The short answer is that neither water activity nor oxygen always “wins.” Freeze-dried treats often fail first by oxidation or humidity-driven texture loss, while jerky often fails first when aw drifts upward and mold risk rises.
Build a pet-treat packaging spec around your real shelf-life engine (not guesswork).

This article uses a “first-failure” approach. The goal is to identify what breaks first, then match packaging barriers and tests to that failure.
What does “shelf life” mean for pet treats: safety, spoilage, or palatability drift?
Shelf life is often treated like one number. That mistake makes “aw vs oxygen” debates meaningless.
In practice, shelf life ends when the first critical failure happens: microbial spoilage, rancidity, or texture drift that reduces acceptance.
Two endpoints that change the answer
| Endpoint | Typical “first failure” | Most useful checks |
|---|---|---|
| Microbial stability | Mold/yeast growth, spoilage odor, surface spots | aw (wettest zone), seal integrity, storage humidity control |
| Quality stability | Rancid notes, aroma dulling, texture softening | Headspace O₂, OTR/MVTR, oxidation marker + sensory checks |
Freeze-dried treats can remain microbiologically stable yet become unacceptable due to aroma loss or texture softening. Jerky can keep “meat aroma” but fail earlier from aw drift that allows mold growth. The practical decision is to define what matters for the SKU: safety margin, palatability, or texture. That definition should be written into the spec as a first-failure rule. It also tells a brand which test cadence is realistic. Microbial endpoints require humidity control and longer observation. Quality endpoints require early oxygen and sensory checkpoints. When a team does not split these endpoints, it often overbuilds the wrong barrier and still gets complaints.
Evidence (Source + Year):
Leistner, L. “Basic aspects of food preservation by hurdle technology.” International Journal of Food Microbiology (2000).
Rahman, M.S. & Labuza, T.P. “Water Activity and Food Preservation.” In Handbook of Food Preservation (2007).
Water activity basics: when aw is the real gate—and when aw is not the limiting factor?
Many teams call a treat “dry” and move on. That shortcut ignores aw, which is about available water, not total moisture.
aw is the strongest predictor of microbial potential, but it does not predict oxidation-driven flavor loss in fatty treats.
aw explains microbial risk, not the whole story
| What aw controls well | What aw does not control well | Common misread |
|---|---|---|
| Mold/yeast likelihood in semi-moist systems | Rancidity rate from oxygen exposure | “Low moisture” = “safe for long shelf life” |
| How humidity pickup changes microbial margin | Aroma loss through film and seals | “If aw is okay, packaging is fine” |
aw is a gate because microbes need available water, but aw is also a moving target during storage. If packaging allows moisture ingress, aw rises over time even when the product started “safe.” That drift is often the real cause of mold claims in jerky-style treats, especially in humid warehouses or long distribution routes. At the same time, aw cannot be used as a proxy for oxidation risk. Fat oxidation depends on oxygen exposure, pro-oxidants, temperature, and time. A freeze-dried salmon cube can have low aw and still lose aroma because oxygen is present and the structure exposes large surface area. A good spec uses aw to set the microbial boundary and uses oxygen control to protect palatability when fats are present.
Evidence (Source + Year):
Labuza, T.P. “Analysis of storage stability of intermediate moisture foods.” NASA Technical Report (1971).
Rahman, M.S. & Labuza, T.P. “Water Activity and Food Preservation.” In Handbook of Food Preservation (2007).
Freeze-dried treats: why porous structure shifts risk toward oxygen + humidity pickup?
Freeze-dried treats look stable because they feel dry and crisp. That surface impression can hide fast quality drift.
Porosity makes freeze-dried treats vulnerable to both oxygen-driven rancidity and humidity-driven texture collapse, so OTR and MVTR often matter together.

Failure mode mapping for freeze-dried fatty treats
| First complaint | Likely driver | Packaging priority |
|---|---|---|
| “Smells stale / fishy / flat” | Oxidation + aroma loss | Low OTR + low headspace O₂ + good seals |
| “Not crunchy anymore” | Humidity pickup | Low MVTR + tight closures + desiccant strategy if needed |
Freeze-dried treats act like sponges because the structure is porous. That structure accelerates moisture sorption and increases contact area for oxygen. If the product contains fats, oxidation can become the first failure even when microbes are inhibited. Research on freeze-dried animal foods shows that lipid oxidation changes with storage conditions and moisture behavior. That pattern is consistent with the real-world complaint sequence: aroma dulls first, then the texture softens if humidity is not controlled. The key design mistake is choosing an “oxygen-focused” strategy while ignoring moisture, or choosing a “moisture-focused” pack while leaving high oxygen in headspace. The better approach is to set a joint target: low headspace oxygen at packing, stable oxygen over time (OTR), and stable dryness over time (MVTR). Seal integrity matters because a small leak can erase both benefits.
Evidence (Source + Year):
Rahman, M.S. et al. “Fat oxidation in freeze-dried grouper during storage at different temperatures and moisture contents.” Food Chemistry (2009).
Uehara, Y. et al. “Lipid Stability and Moisture Behavior of Freeze-dried Meat Treated with Steaming.” Nippon Shokuhin Kagaku Kogaku Kaishi (1985).
Jerky treats: why semi-moist systems are aw-sensitive and mold-prone in real warehouses?
Jerky feels “dry enough,” but many products live in an intermediate-moisture zone that is close to microbial boundaries.
Jerky shelf life often depends on preventing aw drift upward, which means moisture barrier and seal control can matter more than oxygen control in humid routes.
Why jerky fails differently than freeze-dried
| Jerky style | Most common first failure | Most sensitive to |
|---|---|---|
| Lean strips | Mold/yeast + surface spots | Humidity pickup, seal leaks |
| Higher-fat jerky | Rancid notes before mold | Oxygen exposure + temperature |
Jerky is often closer to the “microbial gate” than freeze-dried. Small moisture gains during storage can move aw upward and reduce the margin against mold and yeast. That is why the warehouse climate and distribution humidity matter so much. It is also why seal leaks create outsized damage: a small leak can let the pack equilibrate with humid air, which can shift aw and trigger mold risk even when the product was correctly dried at production. Oxygen still matters, especially for higher-fat formulations, but jerky teams often misallocate effort by chasing “oxygen only” while ignoring MVTR and closure performance. The practical control lever is to decide the primary endpoint: if mold is the first risk, moisture control is the first priority; if rancidity is the first risk, oxygen control becomes the limiter.
Evidence (Source + Year):
Labuza, T.P. “Analysis of storage stability of intermediate moisture foods.” NASA Technical Report (1971).
Leistner, L. “Basic aspects of food preservation by hurdle technology.” International Journal of Food Microbiology (2000).
Which “stops working” first: aw targets, oxygen control, or moisture barrier?
Many failures happen because one control lever drifts while the team watches the wrong metric.
The fastest way to diagnose shelf-life issues is to map the complaint to the driver, then test aw, headspace O₂, and package integrity in the same timeline.
Symptom-to-driver troubleshooting
| Symptom | Most likely driver | Best first test |
|---|---|---|
| Softening / loss of crunch | Moisture ingress (MVTR, leaks) | Weight gain + aw drift + seal leak check |
| Rancid / “cardboard” aroma | Oxidation (oxygen exposure) | Headspace O₂ + oxidation marker + sensory |
| Visible mold spots | aw drift + contamination + oxygen present | aw at surface zone + closure integrity |
Controls stop working in a predictable order. Moisture control often fails first when MVTR is underestimated or seals are inconsistent. Oxygen control often fails first when headspace is not managed at packing, when oxygen ingress is high, or when there is a micro-leak. aw targets stop working when the product equilibrates with humid air, which is usually a packaging and storage problem rather than a “drying” problem. This is why a system view is required. As a flexible packaging manufacturer, we focus on keeping the headspace and product environment stable over time, which means matching film barrier (OTR/MVTR) with the closure and seal window. A pack that looks strong can still fail if seals or zipper tracks let humidity in. A pack that uses strong barrier film can still fail if headspace oxygen starts high and never comes down.
Evidence (Source + Year):
Robertson, G.L. Food Packaging: Principles and Practice, 3rd ed. (2013).
Leistner, L. “Basic aspects of food preservation by hurdle technology.” International Journal of Food Microbiology (2000).
Decision map: which lever to pull first by treat type, fat level, and distribution climate?
Teams often copy a competitor’s bag structure. That approach fails when climate, fat level, and endpoint are different.
A practical decision map starts with “what fails first,” then sets oxygen, moisture, and seal targets in that order.
Simple decision matrix for pet treats
| Scenario | First lever | Packaging focus |
|---|---|---|
| Freeze-dried, fatty, long shelf | Oxygen control | Low OTR + low headspace O₂ + tight seals |
| Freeze-dried, crisp texture priority | Moisture control | Low MVTR + strong closure integrity |
| Jerky, humid distribution | aw stability | Low MVTR + leak-proof seals + stable zipper |
| Jerky, higher-fat formula | Oxygen + temperature | Low OTR + oxygen management + barrier consistency |
The decision map is intentionally simple because teams need repeatable rules. The first lever should be the lever that prevents first failure. If a freeze-dried product loses aroma before anything else, oxygen control is the limiter. If it loses crunch first, moisture control is the limiter. If jerky grows mold first, aw stability through moisture control and seal integrity is the limiter. Fat level matters because it shifts the risk toward oxidation. Distribution climate matters because it shifts the risk toward moisture ingress. A good spec also states what the brand will measure during stability: aw drift, headspace oxygen drift, and pack integrity. Those measurements create a feedback loop that prevents repeated reformulations and repeated bag changes.
Evidence (Source + Year):
Rahman, M.S. et al. “Fat oxidation in freeze-dried grouper during storage at different temperatures and moisture contents.” Food Chemistry (2009).
Robertson, G.L. Food Packaging: Principles and Practice, 3rd ed. (2013).
A simple validation plan: prove shelf life without guessing?
Packaging decisions often rely on “it feels thicker.” That habit leads to expensive failures and slow learning.
A minimal validation plan tracks aw, headspace oxygen, oxidation markers, and seal integrity in one timeline so the team can identify the true limiter.

Minimal test plan that connects cause to complaint
| Metric | Why it matters | Common mistake |
|---|---|---|
| aw (wettest zone) | Microbial margin and drift detection | Only measuring initial aw, not after storage |
| Headspace O₂ | Oxidation risk and barrier performance | Ignoring residual oxygen at packing |
| Oxidation marker + sensory | Links chemistry to real palatability | Testing chemistry without sensory checkpoints |
| Seal/leak verification | Prevents environment drift that defeats barriers | Assuming “no visible leak” means sealed |
A workable plan does not need to be complicated. It needs to be consistent. A team can run a small batch under two packaging conditions and one storage condition that represents the real route. The team should measure aw at the start and after storage to see if humidity pickup is occurring. The team should measure headspace oxygen early to confirm oxygen control at packing and again later to check whether oxygen ingress or leaks are occurring. If the treat is fatty, an oxidation marker paired with sensory checks helps connect numbers to buyer perception. Seal integrity should be verified because leaks erase both moisture and oxygen controls. When these measurements are aligned, the team can stop debating “aw vs oxygen” and start controlling the actual failure engine.
Evidence (Source + Year):
Rahman, M.S. & Labuza, T.P. “Water Activity and Food Preservation.” In Handbook of Food Preservation (2007).
Robertson, G.L. Food Packaging: Principles and Practice, 3rd ed. (2013).
Conclusion
No single lever controls shelf life for all pet treats. Identify the first failure, then set aw, oxygen, and moisture targets that your packaging can actually hold. Contact JINYI to align specs with real routes.
Get a packaging recommendation for freeze-dried or jerky treats
About Me
Brand: Jinyi
Slogan: From Film to Finished—Done Right.
Website: https://jinyipackage.com/
我们的使命:
JINYI is a source factory specializing in flexible packaging. My goal is to deliver reliable, practical, and implementable packaging solutions to brands, allowing clients to achieve more stable quality, clearer delivery times, and packaging structures and printing effects that better match their products, all with less communication effort.
About us:
JINYI is a source manufacturer specializing in custom flexible packaging solutions, with over 15 years of production experience serving food, snack, pet food, and daily consumer brands. We operate a standardized facility with gravure printing lines and HP digital printing systems for both large-volume orders and flexible short runs. From material selection to finished pouches, we focus on process control, repeatability, and real-world performance on shelf, in transit, and at end use.
FAQ
- Is water activity the same as moisture content? No. aw describes available water for microbial activity, while moisture content is total water.
- Why do freeze-dried treats lose aroma even when they stay “dry”? Oxygen exposure can drive oxidation and aroma dulling, especially in fatty treats.
- Why does jerky mold if it was dried correctly? Moisture ingress and seal leaks can raise aw during storage, reducing the microbial safety margin.
- Do I need both oxygen and moisture barrier for freeze-dried treats? Many SKUs do, because porosity makes both oxygen contact and humidity pickup significant.
- What is the fastest way to find the real shelf-life limiter? Track aw drift, headspace oxygen drift, and seal integrity in the same storage timeline.

























